Expression significance and diagnostic value of miR-15a-5p and miR-125a in children with epilepsy

doi:10.19854/j.cnki.1008-2425.2022.01.0002

Abstract

Abstract: Objective To explore the expression levels of microRNA-15a-5p(miR-15a-5p) and miR-125a in children with epilepsy,and analyze their diagnostic value in children with epilepsy.Methods A total of 82 children with epilepsy admitted to our hospital from September 2017 to April 2021 were included as the epilepsy group,and 65 healthy children who received physical examination in our hospital during the same period were selected as the control group.To detect the expression of serum miR-15a-5p,miR-125a and the levels of interleukin-1β(IL-1β),IL-2,and tumor necrosis factor-α(TNF-α) in the two groups.To analyze the correlation between miR-15a-5p,miR-125a and IL-1β,IL-2,TNF-α and the correlation between miR-15a-5p and miR-125a in children with epilepsy.To analyze the relationship between the expression of miR-15a-5p,miR-125a and clinical features in children with epilepsy.A receiver operating characteristic curve(ROC) was drawn to evaluate the area under the curve(AUC) of the two in diagnosing childhood epilepsy.Results The expressions of serum miR-15a-5p and miR-125a in the epilepsy group were lower than those in the control group,and the levels of IL-1β,IL-2,and TNF-α were higher than those in the control group(P＜0.05).Pearson linear correlation test showed that serum miR-15a-5p and miR-125a in children with epilepsy were negatively correlated with IL-1β,IL-2,TNF-α levels,and miR-15a-5p was positively correlated with miR-125a(P＜0.05).The miR-15a-5p and miR-125a low expression group had frequent attacks,the duration of symptoms was more than 10 minutes,and the proportion of generalized attacks was higher than that in the miR-15a-5p and miR-125a high expression group(P＜0.05).The AUC of serum miR-15a-5p and miR-125a alone and combined with the diagnosis of childhood epilepsy were 0.773,0.756,0.862,respectively.Conclusion The expression of serum miR-15a-5p and miR-125a in children with epilepsy is decreased,and their expression is closely related to inflammatory factors,which has a certain diagnostic value for children with epilepsy.

Key words: Epilepsy, MiR-15a-5p, MiR-125a, MicroRNA, Diagnostic value, Correlation

摘要： 目的探讨癫痫患儿微小核糖核酸-15a-5p(miR-15a-5p)、miR-125a的表达水平,分析二者对儿童癫痫的诊断价值。方法纳入我院2017年9月～2021年4月收治的癫痫患儿82例作为癫痫组,取同期于我院体检的健康儿童65例为对照组。检测两组血清miR-15a-5p、miR-125a表达以及白细胞介素-1β(IL-1β)、IL-2、肿瘤坏死因子-α(TNF-α)水平,分析癫痫患儿miR-15a-5p、miR-125a与IL-1β、IL-2、TNF-α的相关性及miR-15a-5p与miR-125a的相关性。分析癫痫儿童miR-15a-5p、miR-125a表达与临床特征的关系,绘制受试者工作特征曲线(ROC)评估二者诊断儿童癫痫的曲线下面积(AUC)。结果癫痫组血清miR-15a-5p、miR-125a表达低于对照组,IL-1β、IL-2、TNF-α水平高于对照组(P＜0.05)。Pearson线性相关检验提示癫痫患儿血清miR-15a-5p、miR-125a与IL-1β、IL-2、TNF-α水平呈负相关,miR-15a-5p与miR-125a呈正相关(P＜0.05)。miR-15a-5p、miR-125a低表达组频繁发作、症状持续时间＞10 min、全面性发作占比高于miR-15a-5p、miR-125a高表达组(P＜0.05)。血清miR-15a-5p、miR-125a单独与联合诊断儿童癫痫的AUC分别为0.773、0.756、0.862。结论癫痫患儿血清miR-15a-5p、miR-125a表达降低,二者表达与炎症因子密切相关,对儿童癫痫有一定诊断价值。

关键词: 癫痫, miR-15a-5p, miR-125a, 微小核糖核酸, 诊断价值, 相关性

CLC Number:

R742.1

Ding Bo, Pang Qiming, Huang Ting. Expression significance and diagnostic value of miR-15a-5p and miR-125a in children with epilepsy[J]. Chinese Journal of Stereotactic and Functional Neurosurgery, 2022, 35(1): 6-11.

丁博, 庞启明, 黄婷. 癫痫儿童miR-15a-5p、miR-125a表达意义及诊断价值分析[J]. 立体定向和功能性神经外科杂志, 2022, 35(1): 6-11.

References

[1] Karrasch M,Tiitta P,Hermann B,et al.Cognitive Outcome in Childhood-Onset Epilepsy:A Five-Decade Prospective Cohort Study[J].J Int Neuropsych Soc,2017,23(4):332~340.
[2] Morris G,O'Brien D,Henshall D C .Opportunities and challenges for microRNA-targeting therapeutics for epilepsy[J].Trends Pharmacol Sci,2021,42(7):605~616.
[3] 吴玉兰,蔡皓然,谢海航,等.MiR-125a-5p靶向APC激活经典WNT信号通路促进hiPSCs分化为多巴胺能神经前体细胞[J].解剖学研究,2020,42(1):8~18.
[4] 张丽,魏文石.MicroRNA与认知功能[J].老年医学与保健,2012,18(2):123~125.
[5] Ma Y .The Challenge of microRNA as a Biomarker of Epilepsy[J].Curr Neuropharmacol,2017,16(1):37~42.
[6] 李哲,王丽辉,陈芳.小儿癫痫的诊断与治疗[M].北京:人民军医出版社,2014:20~21.
[7] Fiest K M,Sauro K M,Wiebe S,et al.Prevalence and incidence of epilepsy:A systematic review and meta-analysis of international studies[J].Neurology,2017,89(6):641~642.
[8] 蔡伦,韦理萍.炎症在慢性癫痫发作中的作用[J].脑与神经疾病杂志,2017,25(4):259~261.
[9] 孟尧,唐重阳,韩志斌,等.MicroRNA与癫痫的研究进展[J].现代生物医学进展,2019,19(6):203~206.
[10] 陈爱玲,刁丽梅.miRNA在癫痫发病机制中的研究进展[J].中国实验诊断学,2018,22(10):1851~1853.
[11] 蔡承魁,田丽颖,韩康,等.CCND1基因3'-UTR区miR-15a-5p和miR-16-5p结合位点的荧光素酶报告载体构建及分析[J].现代肿瘤医学,2017,25(22):3567~3572.
[12] Wang L,Lv Q,Song X,et al.ADRB2 suppresses IL-13-induced allergic rhinitis inflammatory cytokine regulated by miR-15a-5p[J].Human Cell,2019,32(1):306~315.
[13] Li N,Pan J,Liu W,et al.MicroRNA-15a-5p serves as a potential biomarker and regulates the viability and apoptosis of hippocampus neuron in children with temporal lobe epilepsy[J].Diagn Pathol,2020,15(1):46.
[14] 顾浩,冯东福.重型颅脑损伤病人外周血miRNA生物信息学分析[J].中国临床神经外科杂志,2019,24(12):34~36.
[15] Liu Q,Wang L,Yan G,et al.MiR-125a-5p Alleviates Dysfunction and Inflammation of Pentylenetetrazol-induced Epilepsy Through Targeting Calmodulin-dependent Protein Kinase IV(CAMK4)[J].Curr Neurovasc Res,2019,16(4):365~372.
[16] 韦倩娜,林诗映,王玲.炎症因子水平与癫痫患者脑电图异常表现的关系研究[J].中国药物与临床,2019,19(3):464~466.
[17] 李超,郭旗.细胞因子IL-1β、IL-2、IL-6、IL-8和TNF-α水平在癫痫患者外周血中的变化及其意义[J].解放军预防医学杂志,2018,36(3):375~377.
[18] 胡瑞红,江荣雷,买吾拉江·阿木提,等.癫痫患者发作不同时间血清IL-1β和TNF-α水平与脑电图异常的相关性研究[J].现代医学,2018,46(7):796~799.
[19] Abdel H,Kholoussi N M,Eissa E,et al.MicroRNAs as Immune Regulators of Inflammation in Children with Epilepsy[J].Int J Mol Cell Med,2020,9(3):188~197.