Notch3与TIP30表达对脑胶质瘤患者预后评估价值

doi:10.19854/j.cnki.1008-2425.2022.05.0004

摘要/Abstract

摘要： 目的探讨分析Notch3与TIP30表达对脑胶质瘤患者预后评估价值。方法收集2019年12月至2020年12月期间保定市第七医院手术切除经病理科确诊的135例脑胶质瘤患者病理组织标本135份,并取瘤旁组织60份。采用蛋白质印迹法(western blot)和免疫组化法检测脑胶质瘤组织中和瘤旁组织中Notch3和Tat(transactivator of transcription)作用蛋白30(Tat-interactive protein 30,TIP30)表达情况。比较不同组织中Notch3、TIP30阳性率情况及Notch3、TIP30表达与脑胶质瘤临床病理特征的关系。随访术后1年患者生存情况,采用Kaplan -Meier法分析Notch3、TIP30表达与脑胶质瘤患者预后的关系,并采用 Spearman等级相关性分析Notch3与TIP30表达的关系。结果胶质瘤组织中Notch3蛋白表达量(2.25±0.63)高于瘤旁组织的(0.95±0.21),Notch3阳性率57.78%高于瘤旁组织的36.67%,而TIP30蛋白表达量(0.42±0.11)低于瘤旁组织的(0.71±0.23),TIP30阳性率48.15%显著低于瘤旁组织的86.67%(P＜0.05)。胶质瘤组织中Notch3阳性表达与患者患者性别、肿瘤部位、切除范围无关(P＞0.05),与患者年龄、肿瘤直径、病理分级、Ki-67表达有关(P＜0.05);胶质瘤组织中TIP30阳性表达与患者性别、年龄、肿瘤部位、切除范围无关(P＞0.05),与肿瘤直径、病理分级、Ki-67表达有关(P＜0.05)。Cox分析结果显示,病理分级(HR=2.401,95%CI:1.572～3.667)、Ki-67高表达(2.492,95%CI:1.542～4.028)、Notch3高表达(HR=2.221,95%CI:1.138～4.333)、TIP30低表达(HR=8.457,95%CI:2.050～34.886)是影响脑胶质瘤患者预后的重要影响因素(P＜0.05)。Kaplan-Meier生存分析,结果显示,Notch3高表达(评分＜2 分)组脑胶质瘤患者1年生存时间低于低表达(评分＞2 分)组(P＜0.05);TIP30高表达(评分＞3 分)组脑胶质瘤患者1年生存时间高于低表达(评分＜3 分)组(P＜0.05)。Spearman 等级相关性分析,结果发现, 脑胶质瘤组织中Notch3和TIP30表达呈负相关(r= -0.539,P＜0.01)。结论脑胶质瘤患者组织中Notch3呈较高表达,TIP30呈较低表达,随着病理分级增加Notch3表达越高,TIP30表达降低,患者预后较差,其表达对早期病情及预后评估具有重要参考价值。

关键词: Notch3, Tat作用蛋白30, 脑胶质瘤, 病理特征, 预后

Abstract: Objective To explore the prognostic value of Notch3 and TIP30 expression in glioma patients.Methods From December 2019 to December 2020,135 pathological tissue specimens from 135 patients with brain gliomas diagnosed by pathology after surgical resection in Baoding No.7 Hospital were collected,and 60 paratumor tissues were collected.The expression of Notch3 and Tat-interactive protein 30 (TIP30) in glioma tissue and paratumor tissue were detected by western blot and immunohistochemistry.The positive rates of Notch3 and TIP30 in different tissues and the relationship between the expression of Notch3 and TIP30 and the clinicopathological characteristics of glioma were compared.The survival of patients was followed up for 1 year after operation.The Kaplan-Meier method was used to analyze the relationship between the expression of Notch3 and TIP30 and the prognosis of patients with glioma,and the relationship between Notch3 and TIP30 expression was analyzed by Spearman rank correlation.Results The expression of Notch3 protein in glioma tissue (2.25±0.63) was higher than that in paratumor tissue (0.95±0.21),the positive rate of Notch3 was 57.78% higher than that in paratumor tissue(36.67%),and the expression of TIP30 protein (0.42±0.11) The positive rate of TIP30 was 48.15% lower than that of the adjacent tissue (0.71±0.23),which was significantly lower than that of the adjacent tissue (86.67%) (P<0.05).The positive expression of Notch3 in glioma tissue was not related to the patient's gender,tumor location,and resection range(P>0.05),but was related to the patient's age,tumor diameter,pathological grade,and Ki-67 expression (P<0.05).The positive expression of TIP30 was not related to the patient's gender,age,tumor location and extent of resection (P>0.05),but was related to tumor diameter,pathological grade and Ki-67 expression (P<0.05).Cox analysis showed that pathological grade (HR=2.401,95%CI:1.572-3.667),high expression of Ki-67 (2.492,95%CI:1.542-4.028),high expression of Notch3 (HR=2.221,95%CI: 1.138~4.333) and low expression of TIP30 (HR=8.457,95%CI:2.050-34.886) were important influencing factors for the prognosis of glioma patients (P＜0.05).Kaplan-Meier survival analysis showed that the one-year survival time of brain glioma patients in the high Notch3 expression (score < 2 points) group was lower than that in the low expression (score > 2 points) group (P< 0.05);TIP30 high expression (score<2 points) The 1-year survival time of glioma patients in the group with glioma >3 points) was higher than that in the low expression (score <3 points) group (P<0.05).Spearman rank correlation analysis showed that the expression of Notch3 and TIP30 in glioma tissue was negatively correlated (r=-0.539,P<0.01).Conclusion The expression of Notch3 in the tissues of glioma patients is higher,and the expression of TIP30 is lower.With the increase of pathological grade,the expression of Notch3 is higher,the expression of TIP30 is lower,and the prognosis of patients is poor.Its expression has important reference value for early disease and prognosis evaluation.

Key words: Notch3, Tat-acting protein 30, Glioma, Pathological features, Prognosis

中图分类号:

R739.41

杜云, 王卫中, 刘梦, 姚萌萌. Notch3与TIP30表达对脑胶质瘤患者预后评估价值[J]. 立体定向和功能性神经外科杂志, 2022, 35(5): 273-279.

Du Yun, Wang Weizhong, Liu Meng, et al. The prognostic value of Notch3 and TIP30 expression in patients with glioma[J]. Chinese Journal of Stereotactic and Functional Neurosurgery, 2022, 35(5): 273-279.

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