LncRNA PVT1在颅内动脉瘤发生及破裂中的临床意义研究

doi:10.19854/j.cnki.1008-2425.2024.05.0008

摘要/Abstract

摘要： 目的探讨长链非编码人浆细胞瘤转化迁移基因1(lncRNA PVT1)在颅内动脉瘤患者血浆中的表达变化及临床意义。方法选取安庆市中医医院2023年1月至2024年6月住院治疗的30例未破裂颅内动脉瘤患者和30例破裂动脉瘤患者,另选取同期门诊健康体检者30例为对照组;分别采用实时荧光定量PCR(qRT-PCR)检测所有入组人群血浆样本中的LncRNA PVT1表达量,及ELISA方法检测血浆样本中炎症因子IL-8、TNF-α的表达水平;比较健康对照组和颅内动脉瘤组临床资料以及血浆LncRNA PVT1水平,采用受试者工作特征(ROC)曲线分析血浆LncRNA PVT1水平对颅内动脉瘤的评估价值以及对未破裂颅内动脉瘤和破裂颅内动脉瘤的鉴别诊断价值;采用Person分析血浆LncRNA PVT1水平与炎症因子IL-8、TNF-α的相关性。结果颅内动脉瘤患者血浆LncRNA PVT1水平高于对照组,破裂颅内动脉瘤组血浆LncRNA PVT1水平高于未破裂颅内动脉瘤组;颅内动脉瘤患者血浆LncRNA PVT1水平与IL-8、TNF-α表达成正相关,且具有统计学意义(P＜0.05)。ROC曲线显示,血浆LncRNA PVT1预测颅内动脉瘤的AUC为0.808,敏感性为80.0%,特异性为97.8%;血浆LncRNA PVT1鉴别诊断未颅内动脉瘤与破裂颅内动脉瘤的AUC为0.769,敏感性为86.7%,特异性为73.3%。结论颅内动脉瘤患者血浆LncRNA PVT1表达水平的升高与炎症因子及动脉瘤严重程度相关,提示LncRNA PVT1可能通过调节炎症细胞因子的表达水平参与调控颅内动脉瘤的病程进展。

关键词: 颅内动脉瘤, 人浆细胞瘤转化迁移基因1, 炎症因子

Abstract: Objective To investigate the expression changes of long non-coding RNA plasmacytoma variant translocation 1 (lncRNA PVT1) in the plasma of patients with intracranial aneurysms and its clinical significance. Methods A total of 30 patients with unruptured intracranial aneurysms and 30 patients with ruptured aneurysms were selected from Anqing Traditional Chinese Medicine Hospital between January 2023 and June 2024.An additional 30 healthy individuals undergoing routine health check-ups were included as a control group.The expression levels of LncRNA PVT1 in plasma samples from all enrolled subjects were measured using quantitative real-time PCR (qRT-PCR),and the levels of inflammatory factors IL-8 and TNF-α were analyzed using ELISA.Clinical data and plasma LncRNA PVT1 levels were compared between the healthy control group and the intracranial aneurysm group.Receiver operating characteristic (ROC) curve analysis was performed to assess the value of plasma LncRNA PVT1 levels in evaluating intracranial aneurysms and differentiating between unruptured and ruptured aneurysms.The correlation between plasma LncRNA PVT1 levels and inflammatory factors IL-8 and TNF-α was analyzed using Pearson correlation. Results The levels of plasma LncRNA PVT1 in patients with intracranial aneurysms were significantly higher than those in the control group,with the ruptured aneurysm group showing higher levels than the unruptured group.A positive correlation was found between plasma LncRNA PVT1 levels and the expression of IL-8 and TNF-α,which was statistically significant (P＜0.05).The ROC curve showed that the AUC for plasma LncRNA PVT1 in predicting intracranial aneurysms was 0.808,with a sensitivity of 80.0% and specificity of 97.8%.The AUC for differentiating between unruptured and ruptured intracranial aneurysms was 0.769,with a sensitivity of 86.7% and specificity of 73.3%. Conclusion The elevated expression levels of plasma LncRNA PVT1 in patients with intracranial aneurysms are associated with inflammatory factors and the severity of aneurysms.This suggests that LncRNA PVT1 may play a role in the progression of intracranial aneurysms by regulating the expression levels of inflammatory cytokines.

Key words: Intracranial aneurysm, Plasmacytoma variant translocation 1, Inflammatory factors

中图分类号:

R743

陈敏, 李志宏, 唐剑, 查正江. LncRNA PVT1在颅内动脉瘤发生及破裂中的临床意义研究[J]. 立体定向和功能性神经外科杂志, 2024, 37(5): 298-302.

Chen Min, Li Zhihong, Tang Jian, Zha Zhengjiang. Clinical significance of LncRNA PVT1 in the occurrence and rupture of intracranial aneurysms[J]. Chinese Journal of Stereotactic and Functional Neurosurgery, 2024, 37(5): 298-302.

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