泽兰水提取物调控LPS诱导的PC12细胞损伤的分子机制

doi:10.19854/j.cnki.1008-2425.2021.06.0002

摘要/Abstract

摘要： 目的探讨泽兰水提取物对脂多糖(LPS)诱导的PC12细胞增殖、凋亡、炎症反应的影响及其可能作用机制。方法采用LPS诱导的大鼠肾上腺嗜铬细胞瘤PC-12建立细胞损伤模型,加入不同剂量的泽兰水提取物处理细胞,分别将anti-miR-NC、anti-miR-148a-3p转染至LPS诱导的PC12细胞后加入泽兰水提取物处理24 h;采用MTT实验检测细胞增殖;采用流式细胞术检测细胞凋亡率;qRT-PCR法检测miR-148a-3p的表达水平;Western blot法检测Cleaved-caspase-3、IL-1β、IL-6、TNF-α蛋白表达量。结果 LPS诱导的PC12细胞A值及miR-148a-3p的表达水平降低(P＜0.05),凋亡率及Cleaved-caspase-3、IL-1β、IL-6、TNF-α蛋白水平升高(P＜0.05);泽兰水提取物可明显提高A值及miR-148a-3p的表达水平(P＜0.05),降低凋亡率及Cleaved-caspase-3、IL-1β、IL-6、TNF-α蛋白水平(P＜0.05);anti-miR-148a-3p与泽兰水提取物联合处理后可明显逆转泽兰水提取物对LPS诱导的PC12细胞增殖、凋亡及炎症反应的作用。结论泽兰水提取物可通过上调miR-148a-3p而促进增殖及抑制细胞凋亡、炎症反应从而减轻LPS诱导的PC12细胞损伤。

关键词: 泽兰水提取物, miR-148a-3p, 脂多糖, PC12细胞, 增殖, 凋亡

Abstract: Objective To explore the effect of the aqueous extract of Lycopus lycidus Turcz on the proliferation,apoptosis and inflammation of PC12 cells induced by lipopolysaccharide (LPS) and its possible mechanism.Methods The rat adrenal pheochromocytoma PC-12 induced by LPS was used to establish a cell injury model.Different doses of aqueous extract of Lycopus lycidus Turcz were added to treat the cells.Anti-miR-NC and anti-miR-148a-3p were transfected into LPS-induced PC12 cells,and then treated with an aqueous extract of Lycopus lycidus Turcz for 24 h.MTT assay was used to detect cell proliferation.Flow cytometry was used to detect the apoptosis rate.qRT-PCR method to detect the expression level of miR-148a-3p.Western blot method was used to detect the expression of Cleaved-caspase-3,IL-1β,IL-6 and TNF-α protein.Results LPS-induced PC12 cell A value and the expression level of miR-148a-3p were decreased (P＜0.05),apoptosis rate and the protein level of Cleaved-caspase-3,IL-1β,IL-6 and TNF-α were increased (P＜0.05).Aqueous extract of Lycopus lycidus Turcz could significantly increase the A value and the expression level of miR-148a-3p (P＜0.05),reduce the rate of apoptosis and the protein level of Cleaved-caspase-3,IL-1β,IL-6 and TNF-α (P＜0.05).The combined treatment of anti-miR-148a-3p and aqueous extract of Lycopus lycidus Turcz could significantly reverse the effects of Adenophora adenophora water extract on the proliferation,apoptosis and inflammation of PC12 cells induced by LPS.Conclusion Aqueous extract of Lycopus lycidus Turcz could promote proliferation and inhibit cell apoptosis and inflammation by up-regulating miR-148a-3p,thereby reducing the damage of PC12 cells induced by LPS.

Key words: Aqueous extract of Lycopus lycidus Turcz, MiR-148a-3p, Lipopolysaccharide, PC12 cells, Proliferation, Apoptosis

中图分类号:

R446.9

王宁, 于海, 宋千, 廉民学, 鲍刚, 何百祥. 泽兰水提取物调控LPS诱导的PC12细胞损伤的分子机制[J]. 立体定向和功能性神经外科杂志, 2021, 34(6): 326-330.

Wang Ning, Yu Hai, Song Qian, et al.. Aqueous extract of Lycopus lycidus Turcz regulates the molecular mechanism of LPS-induced PC12 cell damage[J]. Chinese Journal of Stereotactic and Functional Neurosurgery, 2021, 34(6): 326-330.

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