关键词: 烟雾病, Olink蛋白质组学, ROBO2, 生物信息学分析

Abstract: Objective To identify differential proteins in serum between moyamoya disease (MMD) patients and healthy controls using Olink targeted proteomics,elucidate their potential roles in disease pathogenesis and progression,and evaluate their value as prognostic biomarkers. Methods We enrolled 43 MMD patients and 43 non-MMD controls admitted to Fuzhou First People's Hospital from January 2023 to May 2025 serum samples were collected,and clinical parameters (blood pressure,blood glucose,blood lipids,oxygen saturation,heart rate) were recorded.Differential proteins were detected using Olink targeted proteomics panels.GO and KEGG enrichment analyses were performed to identify critical signaling pathways. Results MMD patients exhibited significantly elevated blood pressure,blood glucose,blood lipids,and oxygen saturation compared to controls (P<0.05).We identified 30 differentially expressed proteins,with ROBO2 showing the most pronounced upregulation in MMD.Enrichment analysis revealed significant involvement of these proteins in “protein binding,” “signal transduction,” and “cytokine-receptor interaction” pathways.Random forest modeling confirmed that ROBO2 expression level provides optimal predictive power for MMD diagnosis and adverse prognosis. Conclusion The “protein binding–signal transduction–cytokine receptor interaction” network is significantly activated in MMD patient serum.ROBO2 demonstrates marked upregulation and represents a potential biomarker for early diagnosis and prognostic assessment of MMD.

Key words: Moyamoya disease, Olink proteomics, ROBO2, Bioinformatics analysis