毛钩藤碱在脑缺血再灌注损伤动物模型中的保护作用

doi:10.19854/j.cnki.1008-2425.2024.01.0005

立体定向和功能性神经外科杂志 ›› 2024, Vol. 37 ›› Issue (1): 22-27.DOI: 10.19854/j.cnki.1008-2425.2024.01.0005

毛钩藤碱在脑缺血再灌注损伤动物模型中的保护作用

江华, 徐达, 杨雅玲, 李清金

363000 漳州联勤保障部队第九〇九医院(厦门大学附属东南医院)神经内科

收稿日期:2023-09-24 出版日期:2024-02-25 发布日期:2024-04-03
通讯作者: 李清金 2002lqj@163.com
基金资助:
首都医科大学附属北京天坛医院课题项目(编号:HX-A-053(2021))

Protective effect of Hirsutine on animal models of cerebral ischemia-reperfusion injury

Jiang Hua, Xu Da, Yang Yaling, Li Qingjing

Department of Neurology,99th Hospital of Joint Logistic Support Force (Southeast Hospital Affiliated to Xiamen University),Zhangzhou,Fujian 363000,China

Received:2023-09-24 Online:2024-02-25 Published:2024-04-03
Contact: Li Qingjing 2002lqj@163.com

摘要/Abstract

摘要： 目的探究毛钩藤碱(Hirsutine)在大鼠脑缺血再灌注损伤(CIRI)中的神经保护作用及其潜在机制。方法 SD大鼠用于建立大脑中动脉闭塞再灌注(MCAO/R)模型,并将大鼠分为6个实验组(n=6):对照组;模型组;Hirsutine低剂量(5 mg/kg)组;Hirsutine中剂量(10 mg/kg)组;Hirsutine高剂量(20 mg/kg)组;依达拉奉(30 mg/kg,阳性对照)组。取脑组织进行TTC染色,测量脑梗死和神经行为缺陷;HE和Nissl染色检测神经元损伤;Tunel染色检测海马细胞凋亡;ELISA法检测脑组织活性氧(ROS),丙二醛(MDA),超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)水平;蛋白质印迹检测机制相关蛋白的表达水平。结果 Hirsutine治疗显著减轻了MCAO/R大鼠的脑梗死和神经元损伤,并改善了脑神经行为缺陷。Hirsutine有效抑制氧化应激和细胞凋亡。此外,Hirsutine激活了MCAO/R大鼠神经元中的PI3K/Akt/Nrf2通路。结论 Hirsutine通过激活PI3K/Akt/ Nrf2信号通路改善MCAO/R大鼠脑组织氧化应激和细胞凋亡,从而缓解CIRI诱导的神经元损伤。

关键词: 毛钩藤碱, 脑缺血再灌注, 神经损伤, 氧化应激, 凋亡

Abstract: Objective To explore the neuroprotective effect and potential mechanism of Hirsutine in rat cerebral ischemia-reperfusion injury (CIRI). Methods SD rats were used to establish a middle cerebral artery occlusion reperfusion (MCAO/R) model and were divided into 6 groups (n=6):Control group; Model group; Hirsutine low-dose (5 mg/kg) group; Hirsutine medium dose (10 mg/kg) group; Hirsutine high-dose (20 mg/kg) group; Edaravone (30 mg/kg,positive control) group.TTC staining was performed on brain tissue,the cerebral infarction and neurobehavioral defects were measured.HE and Nissl staining were used to detect neuronal damage.Tunel staining was used to detect hippocampal cell apoptosis; ELISA method was used to detect the levels of reactive oxygen species (ROS),malondialdehyde (MDA),superoxide dismutase (SOD),and glutathione peroxidase (GSH Px) in brain tissues.Western blotting is used to detect the expression level of proteins related to the mechanism. Results The results showed that Hirsutine treatment significantly reduced cerebral infarction and neuronal damage in MCAO/R rats,and improved brain neurobehavioral deficits.Hirsutine effectively inhibited oxidative stress and cell apoptosis.In addition,Hirsutine activated the PI3K/Akt/Nrf2 pathway in MCAO/R rat neurons. Conclusion Hirsutine improveed oxidative stress and apoptosis in MCAO/R rat brain by activating PI3K/Akt/ Nrf2 signaling pathway,thereby alleviating CIRI-induced neuronal injury.

Key words: Hirsutine, Cerebral ischemia reperfusion, Nerve injury, Oxidative stress, Apoptosis

中图分类号:

R742

江华, 徐达, 杨雅玲, 李清金. 毛钩藤碱在脑缺血再灌注损伤动物模型中的保护作用[J]. 立体定向和功能性神经外科杂志, 2024, 37(1): 22-27.

Jiang Hua, Xu Da, Yang Yaling, Li Qingjing. Protective effect of Hirsutine on animal models of cerebral ischemia-reperfusion injury[J]. Chinese Journal of Stereotactic and Functional Neurosurgery, 2024, 37(1): 22-27.

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毛钩藤碱在脑缺血再灌注损伤动物模型中的保护作用

Protective effect of Hirsutine on animal models of cerebral ischemia-reperfusion injury

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