关键词: 脑血管支架, 脑梗死, 支架内再狭窄, miR-93-5p, 炎症

Abstract: Objective Predictive value of plasma miR-93-5p was investigated for restenosis after cerebrovascular stent implantation (ISR),as well as its relationship with vasoactive substances and inflammatory cytokines.Methods A total of 132 patients with cerebral infarction who underwent cerebral vascular stent implantation in our hospital and had restenosis during 9-month ultrasonic follow-up were recruited as ISR group,and 124 patients without restenosis evidence were selected as non ISR group according to the ratio of 1:1.The plasma miR-93-5p was detected by real-time fluorescent quantitative PCR.Serum nitric oxide (NO),tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) levels were measured by enzyme-linked immunosorbent assay.Results Plasma miR-93-5p level in ISR group was significantly higher than that in non-ISR group (P< 0.001).Serum NO level in ISR group was significantly lower than that in non-ISR group,and serum TNF-α and IL-6 levels were significantly higher than those in non-ISR group (P＜0.001).The sensitivity,specificity,area under the curve and cut-off of plasma miR-93-5p in diagnosing restenosis after cerebrovascular stent implantation were 70.5%,94.4%,0.873 and 1.254,respectively.Spearman rank correlation coefficient analysis showed that plasmamiR-93-5p level was negatively correlated with NO (r=-0.290,P<0.001),positively correlated with IL-6 (r=0.182,P=0.037) and TNF-α (r=0.207,P=0.017) (P<0.001).Multivariate COX model analysis showed that the increase of plasma miR-93-5p level,stent length > 15mm,serum IL-6 and TNF-α levels were independent risk factors affecting the risk of ISR (P<0.05).Conclusion The plasma miR-93-5p is an independent risk factor for restenosis after cerebrovascular stent implantation,which would be the potential target for the early prevention.

Key words: Cerebrovascular stent, Cerebral infarction, In-stent restenosis, miR-93-5p, Iinflammation