关键词: 保护素D1, 脑缺血再灌注损伤, 炎症反应, 氧化应激, 凋亡

Abstract: Objective To investigate the role ofprotectin D1 on cerebral ischemia reperfusion injury (CIRI) in mice.Methods Ninety C57BL/6 male mice were randomly divided into control group,model group and protectin D1 group.The CIRI model in mice was established by thread embolism method.The mice in the protectin D1 group were injected with 5 mg/kg protectin D1 through the tail vein 30 minutes before ischemia,and the mice in the control group and the model group were given the same amount of normal saline.After 24 hours of reperfusion,the infarct volume,brain water content and blood-brain barrier permeability of mice were measured.The neural function of mice was evaluated by Bederson scoring.The content of interleukin-1β (IL-1β),cyclooxygenase-2 (COX-2),tumor necrosis factor- α (TNF-α),superoxide dismutase (SOD),glutathione (CAT) and malondialdehyde (MDA) in the brain tissue of mice were detected.TUNEL staining was used to evaluate the apoptosis of brain tissue of mice.Results Compared with the control group,the volume of cerebral infarction,brain water content,blood brain barrier permeability,Bederson scoreand adhesion removal time in the model group were significantly increased,and the rotational drop time was significantly decreased (P＜0.05);Compared with the model group,the volume of cerebral infarction,brain water content,blood brain barrier permeability,Bederson score and adhesion removal time in the protectin D1 group were significantly decreased,and the rotational drop time was significantly decreased (P＜0.05).Compared with the control group,the level of IL-1β,COX-2 and TNF-α in the model group were increased significantly (P＜0.05);Compared with model group,the level of IL-1β,COX-2 and TNF-α were significantly decreased (P＜0.05).Compared with the control group,the contents of SOD and CAT in the model group were decreased significantly,while the content of MDA were increased (P＜0.05);Compared with the model group,the contents of SOD and CAT in the protectin D1 group were significantly increased,while the contents of MDA was significantly decreased (P＜0.05).Compared with the control group,the apoptosis index in the model group was increased significantly(P＜0.05);Compared with the model group,the apoptosis index in the protectin D1 group was significantly lower (P＜0.05).Conclusion Protectin D1 alleviates cerebral ischemia reperfusion injury in mice,and its mechanism is related to reducing inflammatory reaction,oxidative stress and apoptosis.

Key words: Protector D1, Cerebral ischemia reperfusion injury, Inflammation, Oxidative stress, Apoptosis