颅内动脉瘤差异表达基因的生信分析及免疫浸润的相关性分析

doi:10.19854/j.cnki.1008-2425.2022.01.0005

摘要/Abstract

摘要： 目的本研究试图通过使用综合生物信息学分析来鉴定颅内动脉瘤的差异表达基因(DEGs) 并揭示其潜在机制。方法利用基因表达综合(GEO)下载GSE13353数据集信息。在DAVID中进行了DEG的基因本体(GO) 功能分析和京都基因和基因组百科全书(KEGG) 通路富集分析。STRING数据库用于评估DEG的相互作用并使用 Cytoscape 软件构建蛋白质-蛋白质相互作用(PPI)网络。使用 CytoHubba 插件选择Hub基因,鉴定枢纽基因网络。利用CIBERSORT评估IA组织中免疫细胞的浸润情况。结果鉴定了93个DEGs,其中有50个上调的基因,43个下调的基因。GO分析结果表明,DEGs在质膜、细胞外区、细胞外间隙细胞内信号转导和免疫反应中显著富集。KEGG通路分析表明,DEGs在趋化因子通路中富集。筛选了前10个中枢基因,包括TLR2、FPR1、CCR1、FCGR3B、CD14、CXCL8、FGR、CD163、AQP9和TNFRSF1B。免疫细胞浸润分析发现滤泡辅助性T细胞、肥大细胞、幼稚B细胞、巨噬细胞 M2和中性粒细胞与IA的破裂有关。结论本研究确定了一系列可能参与颅内动脉瘤进展的关键基因和通路,此外,免疫细胞浸润在IA的发生和发展中起重要作用,为IA破裂的潜在分子机制提供了新的认识。

关键词: 颅内动脉瘤, 生物信息学, 基因

Abstract: Objective Intracranial aneurysm(IA) is a disease caused by weak brain control and is characterized by localized dilation or abnormal bulging of cerebral arteries.This study sought to identify differentially expressed genes(DEGs) in IA and reveal their underlying mechanisms by using comprehensive bioinformatics analysis.Methods We used Gene Expression Omnibus(GEO) to download information from the GSE13353 dataset.Gene Ontology(GO) functional analysis of DEGs and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis were performed in DAVID.the STRING database was used to evaluate DEG interactions and construct protein-protein interaction(PPI) networks using Cytoscape software.Hub genes were selected using the CytoHubba plug-in to identify hub gene networks.CIBERSORT was used to evaluate the infiltration of immune cells in OA tissues.Results Ninety-three DEGs were identified,including 50 up-regulated genes and 43 down-regulated genes.GO analysis showed that DEGs were significantly enriched in plasma membrane,extracellular compartment,extracellular gap intracellular signaling and immune response.KEGG pathway analysis showed that DEGs were enriched in chemokine pathway.The top 10 central genes were screened,including TLR2,FPR1,CCR1,FCGR3B,CD14,CXCL8,FGR,CD163,AQP9,and TNFRSF1B.Immune cell infiltration analysis revealed that follicular helper T cells,mast cells,naive B cells,macrophages M2 and neutrophils were associated with the rupture of IA.Conclusion This study identifies a series of key genes and pathways that may be involved in the progression of intracranial aneurysms.In addition,immune cell infiltration plays an important role in the development and progression of IA,providing new insights into the underlying molecular mechanisms of IA rupture.

Key words: Intracranial aneurysm, Bioinformatics, Genes

中图分类号:

R739.4

刘江, 张云东, 犹秋香. 颅内动脉瘤差异表达基因的生信分析及免疫浸润的相关性分析[J]. 立体定向和功能性神经外科杂志, 2022, 35(1): 24-28.

Liu Jiang, Zhang Yundong, You Qiuxiang. Correlation analysis of differentially expressed genes and immune infiltration in intracranial aneurysms[J]. Chinese Journal of Stereotactic and Functional Neurosurgery, 2022, 35(1): 24-28.

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